پایپس

PIPES (Piperazine-N,N’-bis(2-ethanesulfonic acid)) is a biological buffer widely used in biochemistry and molecular biology. It is a zwitterionic compound that helps maintain a stable pH in a wide range of biological and biochemical experiments, making it an essential component in research involving proteins, nucleic acids, and cellular components.

🏭⚗️ Production
PIPES is synthesized by reacting piperazine with ethanesulfonic acid to form the bis(2-ethanesulfonic acid) derivative. It is commercially available as a white crystalline powder that is highly soluble in water, forming a buffer solution with a pH range ideal for many biological applications.

🔬 Properties
PIPES has the chemical formula C₈H₁₈N₂O₄S₂ and is a zwitterionic compound, meaning it has both positive and negative charges at physiological pH. The buffer range of PIPES is approximately 6.1 to 7.5, making it ideal for maintaining a near-neutral pH in biological and biochemical systems. It is highly soluble in water and does not interfere with most biochemical assays, making it a versatile buffer in laboratory settings.

🧪 Applications
• Cell culture: Used in culture media to maintain pH stability, especially for mammalian cells.
• Protein and enzyme studies: Employed in the stabilization of proteins and enzymes in various biochemical assays.
• Electrophoresis: Frequently used as a buffer in gel electrophoresis, particularly for separating proteins and nucleic acids.
• Biochemical assays: Used in assays to measure enzyme activities or to preserve the activity of cell components.
• Research: Ideal for experiments requiring a pH buffer close to physiological conditions (near neutral pH) such as in the study of cellular processes and molecular interactions.