محلول پوششی ویبریو ولنیفیکوس

Coating Solution for Vibrio vulnificus is a specialized buffer solution used to coat microplate wells or other assay surfaces to immobilize Vibrio vulnificus antigens or antibodies for immunological assays such as ELISA.

🏭⚗️ Production
The coating solution is typically prepared using a carbonate-bicarbonate buffer at pH 9.6, which promotes optimal binding of proteins to polystyrene surfaces. Sometimes additives like Tween-20 (0.05%) or bovine serum albumin (BSA) are included to reduce nonspecific binding. The solution is sterile-filtered and stored at 4°C until use.

🔬 Properties
The solution is clear and colorless with alkaline pH (~9.6). It maintains protein stability and enhances adsorption to microplate surfaces. Its buffering capacity prevents denaturation of coated molecules during the coating process.

🧪 Applications
Used primarily in ELISA and other immunoassays to coat wells with Vibrio vulnificus antigens or antibodies. This immobilization step is critical for sensitive and specific detection of antibodies or antigens related to V. vulnificus infections. It ensures uniform coating and reproducible assay results.

⚠️ Safety
Generally safe for laboratory use but standard precautions should be observed. Avoid ingestion and contact with eyes or skin. Use gloves and handle reagents in a clean environment to prevent contamination. Store coating solution refrigerated and avoid repeated freeze-thaw cycles.