محلول پاپانیکولائوس (OG 6)

Papanicolaou’s Solution (OG‑6) is a specialized cytoplasmic counterstain composed of Orange G dye in alcoholic solution with phosphotungstic acid. It is a key component of the Papanicolaou stain used in cytological examinations.

🏭⚗️ Production
OG‑6 is prepared by dissolving Orange G dye together with phosphotungstic acid in a high‑concentration ethanol solution. The number “6” indicates the specific concentration of phosphotungstic acid. This formulation creates a stable orange-colored solution used in cytology laboratories worldwide.

🔬 Properties
OG‑6 appears as a clear, bright orange liquid that is miscible with water. Its density is approximately 0.83 g/cm³. The solution contains Orange G at a fraction of a percent, combined with phosphotungstic acid to maintain acidity and enhance staining performance. It is flammable due to its high ethanol content and must be handled with caution in a well‑ventilated environment.

🧪 Applications
OG‑6 serves as the first cytoplasmic counterstain in the multichromatic Papanicolaou staining method. It selectively stains keratinized cells and superficial epithelial cells with brilliant orange, facilitating clear contrast when examining vaginal, cervical, and other exfoliative cytology specimens.

⚠️ Safety
OG‑6 is flammable and carries associated hazards. Protective handling protocols including using gloves, eye protection, and proper ventilation are essential. Storage should be at room temperature in a tightly closed container, away from ignition sources.