محلول لیشمن
Leishman Stain is a common histological stain used for the morphological examination of blood smears and bone marrow samples. It is primarily used in hematology for staining blood cells to identify various types of blood disorders and infections. The stain is a combination of eosin and methylene blue, and it is known for providing good contrast for examining cell types in blood.
🏭⚗️ Production
Leishman Stain is prepared by dissolving eosin (a red dye) and methylene blue (a blue dye) in alcohol. The solution is then used to stain blood smears or bone marrow aspirates to differentiate various cell types such as red blood cells, white blood cells, and platelets.
🔬 Properties
Leishman Stain is a pH-sensitive solution, and its staining characteristics depend on the pH of the solution as well as the length of exposure. The stain provides excellent contrast for differentiating the cellular components of blood. When used properly, Leishman stain highlights nucleus and cytoplasmic features, making it easy to identify different types of white blood cells and detect abnormal cells or microorganisms.
🧪 Applications
Blood Smear Staining: Leishman Stain is most commonly used in hematology to stain blood smears for the examination of white blood cells, red blood cells, and platelets. It helps to identify anemia, leukemia, and other blood disorders.
Identification of Parasites: It is also used to detect malaria parasites and other microorganisms in blood samples, making it a valuable tool in infectious disease diagnostics.
Bone Marrow Aspiration: Leishman Stain is employed to stain bone marrow aspirates to assess the condition of hematopoietic cells and detect abnormalities.
