کنگو رد 1%

Congo Red 1.0% is a dye solution primarily used in histology, biochemistry, and microbiology. It is a chemical stain known for its ability to bind to amyloid proteins, making it particularly useful for detecting amyloidosis in tissue samples. Congo Red is also used for bacterial staining and cell viability assays.

🏭⚗️ Production
Congo Red 1.0% is prepared by dissolving Congo Red dye in distilled water to a final concentration of 1.0%. The solution may also contain a small amount of sodium chloride (NaCl) to help maintain the stability of the dye in the solution.

🔬 Properties
Congo Red is a red-colored dye in its dissolved form but exhibits a blue-green birefringence when bound to amyloid under polarized light, which is a key feature for detecting amyloid deposits in tissues. It is soluble in water and exhibits an affinity for amyloid fibers, making it a highly specific stain for amyloidosis. Congo Red also binds to cellulose and some proteins, which allows it to be used in various staining protocols.

🧪 Applications
Amyloid Detection: Congo Red 1.0% is primarily used for detecting amyloid deposits in tissues, a hallmark of amyloidosis. The dye binds to the amyloid fibrils, and under polarized light, the tissue will show a distinctive apple-green birefringence, which confirms the presence of amyloid.
Histology: It is used in tissue staining to highlight cellular structures, especially in biopsy samples suspected of having amyloid-related diseases such as Alzheimer’s disease.
Microbiology: Congo Red is also used in bacterial staining, where it can help identify capsules in bacterial species like Klebsiella and Streptococcus.
Cell Viability Assays: In cell culture experiments, Congo Red is sometimes used in cytotoxicity assays to assess the viability of cells by staining non-viable cells.