محلول رنگ آمیزی گیمسا

Giemsa Stain Solution is a classic Romanowsky-type stain used in histology and cytology for the staining of blood smears, parasites, and chromosomes, providing detailed differentiation of cellular components.

🏭⚗️ Production
Giemsa stain is prepared by dissolving a mixture of methylene blue, eosin, and Azure B dyes in a buffered glycerol-methanol solution. The pH is carefully adjusted (typically around 6.8) to optimize staining quality. The solution is stored in dark bottles to protect from light degradation.

🔬 Properties
The stain is a dark purple to reddish solution that differentially colors cell nuclei, cytoplasm, and other structures. It binds strongly to phosphate groups of DNA and RNA, producing characteristic purple-blue coloration in nuclei and pinkish tones in cytoplasm. The dye components are water-soluble and stable under proper storage.

🧪 Applications
Giemsa stain is extensively used for blood smear examination to identify and differentiate white blood cells, red blood cells, and platelets. It is also vital in detecting blood parasites such as Plasmodium (malaria) and Trypanosoma. In cytogenetics, it aids in chromosome banding techniques.

⚠️ Safety
Giemsa stain contains potentially hazardous chemicals, including methanol and dyes that can cause skin and eye irritation. Use gloves and eye protection during preparation and staining. Work in a well-ventilated area and avoid ingestion or inhalation of powders and vapors. Store in a cool, dark place away from incompatible substances.