محلول رنگ آمیزی کریستال ویولت 1% آبی
Formula | C25H30ClN3 |
Density | 0.99 g/cm3 ( 20 °C) |
Molar mass | 407.99 g/mol |
Bulk density | ~ 220 – 400 kg/m3 |
CAS number | 548-62-9 |
EC index number | 612-204-00-2 |
HS Code | 32041900 |
EC number | 208-953-6 |
Storage | Storage at 15 to 30°C |
SDS | available |
RTECS | BO9000000 |
R phrase | R 45-E22-41-50/53-68 |
S phrase | S 53-26-36/37/39-49-60 |
Odour | odourless |
Form | Liquid |
Color | violet |
Solubility in water | ~ 16 g/l ( 25°C) |
Solubility in ethanol | soluble( 20°C) |
Description | Conforms | |
Identification | Conforms | |
Solubility | Conforms | |
performance test | Conforms |
Crystal Violet Staining Solution 1% is a dye solution used for histological and cytological staining. It is commonly used to stain cellular structures and tissues for examination under a microscope. Crystal violet is a basic dye that binds to negatively charged components of cells, making it ideal for highlighting nucleic acids and cellular structures.
🏭⚗️ Production
Crystal Violet Staining Solution 1% is prepared by dissolving crystal violet dye in distilled water or ethanol to create a 1% concentration. This concentration is typically used for most standard staining procedures.
🔬 Properties
The solution is a purple or violet-colored liquid and has the ability to stain nucleic acids, such as DNA and RNA, as well as certain cellular proteins. Crystal violet has an affinity for acidic structures, making it a powerful stain for cytoplasmic and nuclear components. The staining process is usually fast and can be reversed with a decolorization step if needed.
🧪 Applications
Histology: Crystal Violet Staining Solution 1% is widely used in tissue staining for highlighting cellular structures in fixed tissue sections. It is often used to stain nuclei and cytoplasmic components.
Bacterial Staining: It is also used in microbiology as a part of the Gram staining procedure to stain Gram-positive bacteria.
Cell Culture: In cell culture applications, it can be used to assess cell density and viability by staining cells grown on plates or coverslips.
Cytology: Crystal violet is useful for cytological preparations, particularly for visualizing cell morphology and tissue abnormalities.