محلول رنگآمیزی فوشین پایه
Basic Fuchsin Staining Solution is a vital histological stain used to highlight cellular components by binding to acidic tissue elements, producing vivid magenta coloration in microscopy.
🏭⚗️ Production
This staining solution is typically prepared by dissolving Basic Fuchsin dye in an aqueous solvent, often with added components like ethanol or phenol to enhance staining properties and preserve the solution. The concentration varies depending on the intended application, and the solution is filtered to remove impurities before use.
🔬 Properties
The solution is bright magenta in color, with strong affinity for acidic cellular structures such as nucleic acids and certain cytoplasmic components. Basic Fuchsin acts as a cationic dye that binds to negatively charged molecules, producing high-contrast staining useful for microscopy.
🧪 Applications
Commonly used in histology and microbiology to stain tissues, bacteria, and cellular structures. It is especially valuable in staining acid-fast bacteria (e.g., Mycobacterium species) and in protocols like the Ziehl-Neelsen stain. Also applied in identifying cell walls, nuclei, and cytoplasm in various biological samples.
⚠️ Safety
Handle with care; Basic Fuchsin is potentially toxic and may cause skin and eye irritation. Use gloves and eye protection in a well-ventilated laboratory environment. Avoid ingestion and inhalation. Dispose of waste according to hazardous chemical regulations.
