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Brilliant Crystal Blue Stain Solution (also known as Brilliant Cresyl Blue) is a vital staining dye commonly used in hematology and histology for visualizing reticulocytes and cellular components.

🏭⚗️ Production
This stain is formulated by dissolving Brilliant Cresyl Blue dye (often a zinc chloride complex) in alcohol to create a stable, ready-to-use solution. The final product is filtered and stored in tightly sealed containers to preserve staining quality.

🔬 Properties
Brilliant Crystal Blue appears as a deep blue liquid that is highly soluble in its alcoholic solvent. It is classified as a supravital oxazine dye and binds selectively to ribosomal RNA, enabling sharp contrast and improved microscopic visibility.

🧪 Applications
This solution is extensively used to stain reticulocytes in blood smears, where it highlights the RNA network or punctate structures as dark blue, while other cell elements appear faintly blue to green, facilitating accurate reticulocyte counts. In histology, it may also be applied to visualize nerve tissues, including Nissl bodies, and other cytoplasmic features enhancing cellular differentiation.

⚠️ Safety
Brilliant Crystal Blue should be handled with care due to its alcohol base and staining properties. It can irritate skin or eyes, and its strong color can stain surfaces and clothing. Using gloves, eye protection, and working in a well-ventilated area is recommended.