محلول استاندارد سیترات سالین 20٪

Standard Saline Citrate Solution (SSC 20%) is a concentrated ionic buffer solution widely used in molecular biology for stabilizing nucleic acid hybridization reactions.

🏭⚗️ Production
This solution is prepared by dissolving 175.3 grams of sodium chloride and 88.2 grams of trisodium citrate dihydrate in one liter of distilled water. The pH of the solution is adjusted to 7.0 using hydrochloric acid to ensure optimal buffering conditions. Once prepared, the solution is sterilized by filtration and stored at room temperature for laboratory use.

🔬 Properties
The SSC 20% solution is clear and colorless, with high ionic strength and buffering capacity. It maintains a neutral pH and supports the stability of hydrogen bonds between complementary nucleic acid strands during hybridization processes. The concentrated nature of this solution allows it to be diluted to lower concentrations (such as 2× or 1×) depending on the application.

🧪 Applications
This solution is commonly used in molecular biology procedures such as Southern and Northern blotting, in situ hybridization, and microarray washing steps. It plays a crucial role in facilitating the hybridization and washing of DNA or RNA by maintaining the appropriate salt concentration and pH for strand binding and membrane interactions.

⚠️ Safety
Standard laboratory precautions should be followed when handling this solution. Protective gloves and safety goggles are recommended to prevent skin and eye irritation. The concentrated solution may cause discomfort upon contact, so it should be used in a well-ventilated environment and disposed of according to institutional chemical waste protocols.