مایع رقیق کننده WBC

WBC Diluting Fluid (Turk Solution) is a specialized fluid used in hematology to dilute blood samples before counting white blood cells (WBCs) under a microscope. It is particularly useful in manual cell counting procedures using a hemocytometer. The solution is named after the scientist Turk, who developed the formula.

🏭⚗️ Production
Turk Solution is typically prepared by dissolving a small amount of acetic acid (to lyse red blood cells) and methylene blue (to stain white blood cells) in water. The acetic acid helps to break down red blood cells, leaving behind the white blood cells for easier counting, while methylene blue provides a contrast to the WBCs, making them more visible under the microscope. The concentration of methylene blue is usually very low, ensuring only WBCs are stained, not interfering with other cells.

🔬 Properties
Turk Solution is a colorless or pale yellow solution that, when mixed with blood, lyses red blood cells while preserving the white blood cells. The methylene blue component stains the white blood cells, allowing them to be easily counted and differentiated from the background. The acetic acid in the solution serves to maintain the osmotic balance, which helps preserve the integrity of the WBCs during the dilution process.

🧪 Applications
White Blood Cell (WBC) Counting: The primary application of Turk Solution is in the manual counting of white blood cells in blood samples. By diluting the sample, the solution facilitates the counting of WBCs under a microscope, often using a hemocytometer or Neubauer chamber.
Differentiation of WBCs: The methylene blue in Turk Solution not only stains white blood cells but also helps in differentiating various types of WBCs based on their morphology. This is particularly useful in identifying abnormal or immature white blood cells in blood smears.
Hematological Analysis: Turk Solution is widely used in clinical laboratories for routine hematological analysis, such as in determining the total WBC count, which is important for diagnosing conditions like infections, leukemia, and autoimmune disorders.