لایت گرین

Light Green is a synthetic anionic dye widely used in histology and cytology, especially for staining collagen and other tissue components.

🏭⚗️ Production
Light Green is produced by sulfonation of triphenylmethane dye precursors. It is commonly supplied as Light Green SF Yellowish, either as a powder or pre-prepared aqueous/alcoholic staining solution. It dissolves easily in water and alcohol to form a bright green liquid.

🔬 Properties
The dye appears as a fine green powder or green-blue solution. It has a strong affinity for acidic tissue components and is often used as a counterstain. Light Green SF Yellowish is water-soluble, with good staining intensity and relatively low background staining. It provides sharp contrast when combined with other dyes like eosin or hematoxylin.

🧪 Applications
Light Green is most commonly used in histological staining protocols such as Masson’s Trichrome, where it selectively stains collagen fibers green. It is also used in cytological smears and plant tissue staining to highlight specific structures. In multi-stain systems, it enhances contrast between cellular components.

⚠️ Safety
Light Green dye should be handled with care. While relatively low in toxicity, it may cause irritation to eyes, skin, or respiratory tract if inhaled or in contact with mucous membranes. Use appropriate personal protective equipment and store in a sealed container away from direct light and moisture.