فرمالین 4% فیکساتیو (تیشوفیکس 4)

Formalin 4% Fixative (TishoFix 4) is a widely used tissue fixative solution that is composed of formaldehyde dissolved in water, typically at a concentration of 4%. This fixative is essential in histology and pathology for preserving tissue specimens, maintaining their cellular structure, and preventing enzymatic degradation. It is commonly used for preparing samples for further examination, such as histopathological studies, immunohistochemistry, and molecular analysis.
🏭⚗️ Production
TishoFix 4 is prepared by diluting formaldehyde (37-40%) in distilled water to achieve a 4% concentration. The formaldehyde acts as the active fixing agent, crosslinking proteins and preserving the tissue structure. The solution is usually buffered to a pH range of 7.0-7.4 to maintain optimal fixation and to prevent acidic degradation of cellular components.
🔬 Properties
Formalin 4% (TishoFix 4) is a colorless, aqueous solution with a characteristic pungent odor. The formaldehyde in the fixative works by crosslinking proteins, especially by reacting with amino groups, thus stabilizing the tissue and preventing enzymatic activity. The 4% concentration ensures that the tissue is preserved without over-hardening, which is important for subsequent processing, sectioning, and staining of the samples. Formalin 4% is widely favored for its mild fixing properties compared to more concentrated formaldehyde solutions, which can be too harsh on delicate tissues.
🧪 Applications
Routine Tissue Fixation: TishoFix 4 is commonly used in histology to fix a variety of tissue specimens, including organs, biopsies, and cell cultures. The formaldehyde in the solution preserves the tissue architecture and prevents tissue autolysis, making it suitable for light microscopy.
Preservation for Histopathology: It is widely used in pathological examinations to preserve tissue samples, especially for histopathological staining (e.g., H&E staining), allowing accurate examination of cellular structures and tissue morphology.
Immunohistochemistry: Formalin 4% is frequently used in immunohistochemistry to fix tissues while preserving antigenicity. It maintains the integrity of cell surface markers and intracellular proteins, allowing for the detection of specific biomarkers.
Molecular Analysis: TishoFix 4 is used for preserving tissues intended for molecular biology techniques, such as RNA, DNA, and protein analysis, as it prevents degradation of these biomolecules during the fixation process.
Clinical Use: It is also employed in clinical laboratories for the fixation of biopsy specimens and tissue samples for diagnostic purposes, including cancer diagnosis and tissue screening.