سالین بافر فسفات دولبکو (DPBS)

Dulbecco’s Phosphate Buffered Saline (DPBS) is a balanced salt solution widely used in cell culture and molecular biology to maintain osmotic balance and provide essential ions to cells.

🏭⚗️ Production
DPBS is prepared by dissolving precise amounts of sodium chloride (NaCl), potassium chloride (KCl), sodium phosphate dibasic (Na₂HPO₄), potassium phosphate monobasic (KH₂PO₄), calcium chloride (CaCl₂), and magnesium chloride (MgCl₂) in distilled water. The solution is adjusted to a physiological pH of approximately 7.4 and sterilized by filtration or autoclaving.

🔬 Properties
DPBS is a clear, colorless, isotonic solution with a pH around 7.4. It contains phosphate ions which act as a buffering system to maintain stable pH. The presence of calcium and magnesium ions supports cellular functions such as adhesion and enzyme activity.

🧪 Applications
DPBS is commonly used in cell culture for washing cells, diluting substances, and preparing reagents. It maintains the osmolarity and pH suitable for cells, supports enzyme activities, and helps in maintaining cell viability during manipulations. It is also used in immunological assays and molecular biology protocols.

⚠️ Safety
DPBS is generally safe for laboratory use but should be handled using standard aseptic techniques to prevent contamination. Avoid ingestion or contact with eyes. Store sterile DPBS at room temperature or refrigerated, tightly sealed to maintain sterility.