سافت دیکلسیفیکیشن ( کلسی اوت)

Soft Decalcification (CalciOut) is a gentle decalcifying solution designed for removing calcium deposits from biological tissues while preserving cellular and tissue morphology. It is commonly used in histology and pathology laboratories for preparing bone and calcified tissue samples.

🏭⚗️ Production
CalciOut is formulated by blending mild acids such as ethylenediaminetetraacetic acid (EDTA) or weak organic acids in buffered aqueous solutions. The composition is optimized to slowly dissolve calcium salts without damaging proteins or nucleic acids. The solution is sterile filtered and packaged in airtight containers to maintain efficacy.

🔬 Properties
CalciOut is a clear to slightly cloudy aqueous solution with a mild acidic pH, usually around 4.5 to 6.0. It acts by chelating calcium ions, effectively softening and removing calcified deposits. The solution is gentle, non-corrosive, and preserves the structural integrity of soft tissues and cellular components.

🧪 Applications
CalciOut is widely used in soft decalcification of bone biopsies, teeth, and calcified tissue sections before histological processing. It is favored when subsequent immunohistochemistry or molecular analyses are required, as it minimizes tissue damage compared to strong acid decalcifiers.

⚠️ Safety
CalciOut is generally safe to handle but should be used with standard laboratory precautions. Avoid contact with skin and eyes; wear gloves and eye protection. Store in a cool, dry place away from incompatible substances. Dispose of according to local regulations.