دی کلرومتان برای سنتز DNA و پپتید
| Formula: | CH2Cl2 |
| Density: | 1.33 g/cm3 (20 °C) |
| Molar mass: | 84.93 g/mol |
| CAS number: | 75-09-2 |
| EC index number: | 602-004-00-3 |
| HS code: | 29031200 |
| EC number: | 200-838-9 |
| Storage (temperature): | Store at +15°C to +30 °C |
| SDS | available |
| RTECS: | PA8050000 |
| R phrase: | R 40 |
| S phrase: | S 23.2-24/25-36/37 |
| Odour: | seweetish |
| Form: | liquid |
| Color: | colourless |
| Explosion limit: | 13 -22 Vol % |
| Ignition temperature: | 605 °C |
| Solubility in water: | 20 g/l (20 °C) |
| Boiling point: | 40 °C (1013 hPa) |
| Melting point: | -95 °C |
| Vapour pressure: | 475 hPa (20 °C) |
| Evaporation number: | 1.9 °C |
| Thermal decomposition: | > 120 °C |
| Viscosity dynamical: | 0.43 mPa*s (20 °C) |
| Saturation concentration: | 1549 g/m3 (20 °C) |
| Purity | ≥ | 99.9 | % |
| Solubility | Conforms | ||
| Description | Conforms | ||
| Identification | Conforms | ||
| Refractive index | 1.421 to 1.425 | ||
| Residue after evaporation | ≤ | 0.0005 | % |
| Water | ≤ | 0.005 | % |
Dichloromethane for DNA & Peptide synthesis is a high-purity, anhydrous solvent widely used in peptide and oligonucleotide synthesis, as well as in preparative and analytical procedures in pharmaceutical and biochemical laboratories.
🏭⚗️ Production
The solvent is produced by high-purity distillation of methylene chloride under strictly controlled conditions to remove water and impurities. It is then packaged in sealed, moisture-resistant containers to preserve its anhydrous nature and ensure consistent performance for regulated laboratory and industrial environments.
🔬 Properties
Dichloromethane is a clear, colorless liquid with a low boiling point and moderate density. It is highly volatile, has good solubility for many organic compounds, and is slightly soluble in water. The solvent is chemically stable under normal conditions but can react with strong bases and oxidizing agents. Its low polarity makes it an ideal solvent for extractions, washing, and preparative chromatography in DNA and peptide synthesis.
🧪 Applications
In pharmaceutical and analytical laboratories, dichloromethane is used for extraction and purification steps in peptide and oligonucleotide synthesis workflows. It serves as a solvent for preparative chromatography and low-UV-absorbing washes. It is also used to dissolve or suspend reagents and intermediates during synthetic reactions, and in laboratory-scale reactions where a non-polar, volatile, and stable solvent is required to achieve reproducible and high-purity results.
⚠️ Safety
Dichloromethane is highly volatile, flammable, and can be harmful if inhaled or absorbed through the skin. It should be handled in a well-ventilated fume hood with gloves, protective eyewear, and lab coats. Containers should be stored tightly sealed in a cool, dry place, away from heat and strong oxidizers. Waste and empty containers must be disposed of according to laboratory hazardous waste regulations.


