تریس بافر سالین (TABS) pH 7.5

Tris Buffered Saline (TABS) pH 7.5 is a buffered saline solution widely used in biochemical and molecular biology applications to maintain a stable pH and ionic environment.

🏭⚗️ Production
This solution is prepared by dissolving Tris base (typically 3.03 g/L for 25 mM) and sodium chloride (8 g/L for 137 mM) in distilled water. The pH is carefully adjusted to 7.5 using hydrochloric acid (HCl) or sodium hydroxide (NaOH) to ensure optimal buffering capacity. The solution is then sterilized by filtration and stored at room temperature.

🔬 Properties
The TABS solution is clear and colorless with stable buffering capacity at pH 7.5. It maintains isotonic conditions and provides a suitable environment for enzymatic reactions, protein assays, and immunological techniques.

🧪 Applications
Widely used as a washing buffer and diluent in Western blotting, ELISA, immunohistochemistry, and other biochemical assays. It helps maintain protein stability and reduces nonspecific interactions by providing a controlled pH and ionic strength.

⚠️ Safety
Handle with standard laboratory precautions. Use gloves and eye protection to avoid contact. Store the solution properly and dispose of waste according to institutional safety guidelines.