تریتون X100

Triton X-100 is a non-ionic surfactant widely used in laboratories and industrial applications for its ability to solubilize membranes and proteins without denaturing them.

🏭⚗️ Production
Triton X-100 is produced by the ethoxylation of octylphenol, resulting in a mixture of compounds with polyethylene oxide chains attached to an alkylphenol hydrophobic group. It is manufactured as a clear, viscous liquid with consistent quality and purity for laboratory use.

🔬 Properties
Triton X-100 is a colorless to pale yellow liquid, soluble in water and organic solvents. It has a high hydrophilic-lipophilic balance (HLB) value (~13.5), making it effective at disrupting lipid-lipid and lipid-protein interactions. It is stable across a broad pH range and temperature, with low toxicity compared to ionic detergents.

🧪 Applications
Triton X-100 is commonly used in molecular biology and biochemistry for cell lysis, membrane protein extraction, and permeabilization in immunostaining protocols. It also serves as an emulsifier, dispersant, and cleaning agent in various industrial processes.

⚠️ Safety
While generally considered low in toxicity, Triton X-100 can cause skin and eye irritation. Avoid ingestion and prolonged skin contact. Use gloves and eye protection when handling. Dispose of according to local environmental regulations. Store in a cool, dry place away from strong oxidizers.