بیسمارک براون Y
Formula | C18H18N8.2HCl |
Chemical formula | C18H18N8.2HCl |
molar mass | 419.31 g/mol |
CAS number | 10114-58-6 |
HS Code | 32041900 |
Storage | Store at 15 to 30°C |
SDS | available |
Form | powder |
Dye content | ≥ | 50 | % |
Identification | Conforms | ||
Absorption ʎ max | 454 to 460 | nm | |
Color of solution | Dark red to dark | ||
brown red | |||
TLC -test | Conforms | ||
Suitability for microscopy | Conforms |
Bismarck Brown Y is a basic azo dye used in histology and microbiology for staining tissues, bacteria, and cellular components.
🏭⚗️ Production
Bismarck Brown Y is synthesized through diazotization and coupling reactions involving aromatic amines. It is typically supplied as a brownish-orange powder, which is dissolved in water to prepare staining solutions of various concentrations.
🔬 Properties
This dye appears as a reddish-brown to yellow-brown powder and forms a brown solution when dissolved. It has good solubility in water and binds strongly to acidic components in tissues and cells. Bismarck Brown Y is classified as a basic (cationic) dye and is generally stable under normal storage conditions.
🧪 Applications
Bismarck Brown Y is used in histological staining procedures, such as staining mucins, cartilage, and nuclei. It is also employed in microbiology for staining acid-fast bacteria and as a counterstain in Gram staining. In botanical microscopy, it highlights lignified cell walls and plant structures effectively.
⚠️ Safety
This dye should be handled with appropriate safety precautions. Prolonged exposure or inhalation of powder may cause irritation. Use gloves, protective eyewear, and avoid ingestion or contact with eyes and skin. Store in a tightly sealed container in a dry, cool environment.
