بافر سیترات pH 6.0
Citrate Buffer pH 6.0 is an aqueous solution commonly used to maintain a stable pH of 6.0 in biological and chemical experiments.
🏭⚗️ Production
Citrate buffer is prepared by mixing citric acid and its conjugate base, sodium citrate, in precise ratios to achieve the desired pH of 6.0. This buffer system is widely used due to its good buffering capacity around this pH range.
🔬 Properties
The buffer solution typically has a pH of 6.0 ± 0.05, with good ionic strength and stability. It is colorless, transparent, and miscible with water. The buffering action results from the equilibrium between citric acid and citrate ions.
🧪 Applications
Citrate buffer pH 6.0 is used extensively in biochemical assays, molecular biology protocols, and pharmaceutical formulations. It stabilizes enzymes and proteins, helps maintain the integrity of nucleic acids, and is commonly employed in immunohistochemistry and histology for antigen retrieval.
⚠️ Safety
Citrate buffer is generally safe to handle; however, standard laboratory precautions should be followed. Avoid contact with eyes and prolonged skin exposure. Store in a clean, closed container to prevent contamination.
