بافر سالین فسفات (0.02 مولار، pH 7.3 -7.4)

Phosphate Saline Buffer (0.02 M, pH 7.3–7.4) is a commonly used isotonic buffer solution designed to maintain physiological pH and osmolarity in biological and biochemical experiments.

🏭⚗️ Production
This buffer solution is typically prepared by dissolving sodium chloride, sodium phosphate salts (monobasic and dibasic), and potassium chloride in distilled water. The pH is carefully adjusted to between 7.3 and 7.4 to mimic physiological conditions. The final molarity of phosphate salts is approximately 0.02 M.

🔬 Properties
The solution is clear and colorless with excellent buffering capacity near neutral pH. It maintains osmotic balance and stabilizes biological samples by mimicking the ionic composition of bodily fluids. It is isotonic, preventing cell shrinkage or swelling.

🧪 Applications
Widely used in cell culture, immunohistochemistry, and molecular biology as a washing buffer, diluent, and reagent medium. It helps preserve cell viability and structural integrity during experiments.

⚠️ Safety
Generally safe for laboratory use when handled with standard precautions. Use gloves and eye protection. Avoid ingestion and skin or eye contact. Dispose of according to safety regulations.