بافر تریس ادتا تریتون X100 (TET)

Tris EDTA Triton X-100 (TET) Buffer is a commonly used buffer solution in molecular biology and biochemistry that combines buffering agents and a non-ionic detergent to facilitate cell lysis and protein extraction.

🏭⚗️ Production
This buffer is prepared by dissolving Tris base and EDTA (ethylenediaminetetraacetic acid) in distilled water to achieve the desired molar concentrations, typically 10 mM Tris and 1 mM EDTA. The pH is adjusted to 7.4 using hydrochloric acid or sodium hydroxide. Triton X-100 is then added at a concentration of 0.1% to 1% (v/v), and the solution is mixed thoroughly until homogeneous. The buffer is sterilized by filtration and stored at room temperature or refrigerated depending on use.

🔬 Properties
The TET buffer is clear and colorless, maintaining a stable pH and providing chelation of divalent metal ions through EDTA. Triton X-100 acts as a mild non-ionic detergent, disrupting lipid membranes and aiding in cell lysis without denaturing proteins.

🧪 Applications
This buffer is widely used for cell lysis, protein extraction, and preparation of samples for Western blotting, immunoprecipitation, and other biochemical assays. It helps solubilize membrane proteins while preserving protein function.

⚠️ Safety
Handle with care; Triton X-100 can cause skin and eye irritation. Use gloves, eye protection, and work in a well-ventilated area. Avoid ingestion and inhalation. Dispose of waste according to hazardous chemical regulations.