بافر الکتروفورز TAE 10 x

TAE Electrophoresis Buffer 10X is a widely used buffer solution in molecular biology, specifically for agarose gel electrophoresis of nucleic acids. It is a concentrated stock solution that is diluted to 1X before use in electrophoresis experiments. TAE buffer provides an optimal environment for the migration of DNA and RNA during electrophoresis.

🏭⚗️ Production
TAE buffer is typically prepared by dissolving Tris base, acetic acid, and EDTA (Ethylenediaminetetraacetic acid) in water. The 10X concentrate is made by combining these components at ten times the final desired concentration. Before use in electrophoresis, it is diluted with distilled water to the standard 1X concentration.

🔬 Properties
TAE Electrophoresis Buffer has the chemical formula C₄H₁₁NO₃·C₂H₄O₂ (for the Tris and acetic acid components) and is available as a colorless solution with a slightly acidic pH. The 10X stock solution has a pH of about 8.0, and when diluted to 1X, it maintains a pH of approximately 8.3-8.5. The presence of EDTA helps in chelating divalent cations that could interfere with the stability of DNA or RNA. It is especially suitable for the electrophoresis of large DNA fragments.

🧪 Applications
• Agarose gel electrophoresis: Used as the running buffer for DNA and RNA separation in agarose gel electrophoresis, allowing for high resolution of nucleic acids.
• Nucleic acid analysis: Commonly used in experiments to analyze DNA and RNA samples, including gene sequencing, cloning, and restriction enzyme analysis.
• Molecular biology research: Essential in procedures like PCR analysis, gel purification, and the detection of nucleic acids.
• DNA extraction and purification: TAE buffer helps preserve the integrity of DNA during extraction processes.