آمونیاک HPLC
| Formula | NH3 |
| Chemical formula | NH3 |
| Density | 0.91 g/cm3 (20°C) |
| Molar mass | 17.03 g/mol |
| CAS number | 1336-21-6 |
| HS Code | 28142000 |
| EC number | 215-647-6 |
| Storage | Store at 2 to 25 °C |
| SDS | available |
| R Phrase | R 34-50 |
| S Phrase | S 26-36/37/39-45-61 |
| Odour | pungent |
| Form | liquid |
| Color | colorless |
| Explosion limit | 15.4 – 33.6 vol% |
| Solubility in water | soluble (25°C) |
| Boiling point | 24.7 °C |
| Melting point | _91.5 °C |
| Vapour pressure | ~837 hpa |
| Assay | ≥ | 25.0 | % |
| Description | Conforms | ||
| Solubility | Conforms | ||
| Identification | Conforms | ||
| Color | ≤ | 10 | Hazen |
| Residue on ignition | ≤ | 10 | ppm |
| Aluminum | ≤ | 0.5 | ppm |
| Calcium | ≤ | 0.5 | ppm |
| Copper | ≤ | 0.1 | ppm |
| Iron | ≤ | 0.1 | ppm |
| Potassium | ≤ | 0.5 | ppm |
| Magnesium | ≤ | 0.1 | ppm |
| Sodium | ≤ | 0.5 | ppm |
| HPLC gradient suitability test | ≤ | 10 | ppm |
| (220,254 & 280 nm) chromophoric | |||
| impurities on 4-hydroxyacetophenon |
Ammonia for HPLC is a high‑purity, analytical‑grade reagent and solvent used in pharmaceutical and analytical laboratories for HPLC mobile phase preparation, pH adjustment, and as a modifier in chromatographic separations.
🏭⚗️ Production
Prepared from ultra‑pure ammonia gas dissolved in ultrapure water or suitable organic solvents under strictly controlled conditions to minimize trace metals and contaminants. Standardized for concentration, pH, and solution stability. Supplied in chemically resistant, tightly sealed containers to prevent contamination, moisture uptake, or degradation.
🔬 Properties
A colorless gas or aqueous solution with a pungent odor, highly soluble in water and polar organic solvents. Exhibits basic properties suitable for pH adjustment in reversed-phase and ion-exchange HPLC, minimal UV absorption, and chemical stability under normal laboratory conditions. Corrosive to metals at high concentrations; reacts with acids and oxidizers. Store in a cool, dry, well-ventilated area in appropriate containers.
🧪 Applications
Used in pharmaceutical and analytical laboratories for preparing HPLC mobile phases, controlling pH in aqueous and mixed solvent systems, and enhancing reproducibility of chromatographic separations. Supports ion-pairing, gradient, and reversed-phase separations while maintaining low background interference. Solutions and equipment should be handled carefully to preserve analytical-grade purity.
⚠️ Safety
Corrosive and irritating; can cause burns to eyes, skin, and respiratory tract. Highly toxic in concentrated form. Use in a well-ventilated area with appropriate protective equipment including gloves, goggles, and lab coat. Avoid ingestion and contact with strong acids or oxidizers. Spills should be neutralized and cleaned up according to institutional and environmental safety guidelines. Store in a tightly closed, chemically resistant container in a cool, dry, well-ventilated area away from incompatible substances.



