Wright stain
| HS Code | 32041900 |
| Storage | at +15 to 30 °C |
| SDS | available |
| RS | H225/H301 |
| Ignition temperature | 455 °C |
| Description | Conforms | ||
| Identification | Conforms | ||
| Solubility | Conforms | ||
| Appearance of solution | Clear | ||
| Colour | Dark green | ||
| p H | 6.75 – 6.95 | ||
| Application test | Conforms |
Wright stain is a histologic stain that is widely used to differentiate blood cell types in blood smears and bone marrow samples. It is a type of Romanowsky stain, composed of a mixture of eosin (acidic dye) and methylene blue (basic dye), allowing differential staining of cellular components.
🏭⚗️ Production
Wright stain is prepared by dissolving eosin Y and methylene blue dyes in a methanol solvent, which also acts as a fixative. The dyes are carefully balanced to produce characteristic staining properties. Commercial preparations are available, but it can also be prepared in laboratories following standard recipes.
🔬 Properties
This stain produces a range of colors: eosin stains cytoplasm and hemoglobin shades of pink to red, while methylene blue stains nuclei and basophilic granules blue to purple. The solution is stable, alcohol-based, and can fix cells while staining simultaneously. It is soluble in methanol and other alcohols and typically used in thin films or smears.
🧪 Applications
• Hematology: Commonly used to stain peripheral blood smears and bone marrow aspirates to identify and differentiate white blood cells, red blood cells, and platelets.
• Pathology: Useful for diagnosing blood disorders, infections, and leukemias by visualizing morphology and cellular details.
• Cytology: Applied in bone marrow and blood cytology for detailed cell examination.
• Research: Utilized in laboratories for studying blood cell morphology and function.
