Water for HPLC
| Formula (Hill): | H2O |
| Density: | 1.00g/cm3 (20 °C) |
| Molar mass: | 18.02 g/mol |
| CAS number: | 7732-18-5 |
| HS code: | 28510010 |
| EC number: | 231-791-2 |
| Storage : | Store at +5 to +30 °C |
| SDS | Available |
| RTECS: | ZC0110000 |
| Odour: | Odourless |
| Form: | Liquid |
| Color: | Colourless |
| Boiling point: | 100 °C |
| Melting point: | 0 °C |
| Vapour pressure: | 23hpa (20 °C) |
| Viscosity dynamical: | 0.952 mPa*s (20 °C) |
| Description | Conforms | ||
| Appearance | Conforms | ||
| Abs@200nm | ≤ | 20.0 | mAU |
| Abs@210nm | ≤ | 5.0 | mAU |
| Abs@254nm | ≤ | 0.5 | mAU |
| Abs@300nm | ≤ | 0.005 | mAU |
| Ammonia | Conforms | ||
| Chloride | Conforms | ||
| Calcium | Conforms | ||
| Sulfate | Conforms | ||
| Oxidizable substances | Conforms | ||
| Magnesium | Conforms | ||
| Nitrate | Conforms | ||
| Evaporation residue | ≤ | 5.0 | mg/I |
| Gradient@210nm | ≤ | 5.0 | mAU |
| Gradient@254nm | ≤ | 0.5 | mAU |
| Spec Conductance@25 | ≤ | 1.0 | μS/cm |
| p H@25 | 5.0 – 7.0 |
Water for HPLC is an ultra-high-purity solvent specifically produced for chromatographic and analytical applications requiring extremely low levels of organic, inorganic, and particulate impurities. It is widely used as a primary solvent in reversed-phase HPLC, LC-MS, and high-sensitivity biochemical analyses.
🏭⚗️ Production
Produced through advanced purification processes such as double distillation, deionization, reverse osmosis, sub-micron filtration, and UV oxidation to remove trace organics, ions, bacteria, and particulates. Meets strict HPLC-grade specifications including very low conductivity, low total organic carbon (TOC), and minimal UV absorbance. Packaged in sterile, inert, airtight containers to prevent contamination, microbial growth, or leaching from packaging materials during storage and handling.
🔬 Properties
A clear, colorless, odorless liquid with exceptionally low impurity levels. Fully miscible with a wide range of polar organic solvents such as methanol and acetonitrile. Exhibits excellent UV transparency and chemical compatibility for HPLC and LC-MS applications. Stable under recommended conditions but can absorb atmospheric CO₂, leading to pH changes if exposed. Store in tightly closed containers in a clean, cool environment, protected from contamination.
🧪 Applications
Widely used as a mobile phase component in reversed-phase HPLC and LC-MS due to its high purity and low background signal. Essential for dilution of buffers, standards, and samples in analytical workflows. Applied in sample preparation, reagent formulation, extraction processes, and analytical methods where minimal interference and consistent chromatographic performance are required. Ensures reproducibility and sensitivity in high-resolution chromatographic and spectrometric analyses.
⚠️ Safety
Generally considered non-hazardous under normal laboratory use. However, contamination risks require careful handling to maintain purity. Use clean glassware, avoid contact with unclean surfaces, and keep containers tightly closed when not in use. Handle with standard laboratory PPE including gloves and lab coat to prevent sample contamination rather than for personal protection. Store away from chemicals that may leach or release vapors that could compromise water purity.
