Tris Buffered saline (TABS ) With 1% Tween

Tris Buffered Saline (TABS) with 1% Tween is a buffered salt solution containing a non-ionic detergent, commonly used in immunological and biochemical applications to reduce non-specific binding and background noise.

🏭⚗️ Production
This solution is prepared by dissolving Tris base (typically 24.2 g/L for 0.2 M) and sodium chloride (8 g/L for 0.137 M) in distilled water. The pH is adjusted to 7.4 using hydrochloric acid. After buffering, 1% (v/v) Tween 20, a non-ionic surfactant, is added to the solution. The final mixture is stirred until completely homogeneous and sterilized by filtration if required.

🔬 Properties
The solution is clear and colorless, with a neutral to slightly basic pH. The presence of Tween 20 enhances the solution’s ability to block hydrophobic interactions, reducing background binding in assays. Tris provides stable pH buffering, while sodium chloride maintains isotonic conditions.

🧪 Applications
TBS with 1% Tween is widely used as a washing buffer in Western blotting, ELISA, and immunohistochemistry. It helps remove unbound antibodies or probes while minimizing non-specific interactions, leading to cleaner and more specific results.

⚠️ Safety
This solution is generally safe to handle with standard laboratory precautions. However, prolonged skin contact with Tween 20 may cause mild irritation. Use gloves and eye protection, and handle in a well-ventilated area. Dispose of the solution according to institutional chemical safety protocols.