TBE Electrophoresis buffer 10X

TBE Electrophoresis Buffer 10X is another commonly used buffer solution for agarose gel electrophoresis, specifically for the separation of nucleic acids like DNA and RNA. It is a concentrated solution that is diluted to a 1X working concentration before use. TBE is often preferred for electrophoresis of small DNA fragments and for experiments requiring high resolution.

🏭⚗️ Production
TBE buffer is typically prepared by dissolving Tris base, boric acid, and EDTA (Ethylenediaminetetraacetic acid) in water. The 10X concentrate is made by combining these components at ten times the desired final concentration. Before use, the 10X stock solution is diluted with distilled water to create a 1X working solution.

🔬 Properties
TBE Electrophoresis Buffer has the chemical formula C₄H₁₁NO₃·C₆H₁₁BO₃ (for Tris, boric acid, and EDTA). It is a clear, colorless solution with a slightly basic pH. The 10X stock solution has a pH around 8.3, and the 1X solution has a pH of about 8.0-8.5. The EDTA in TBE helps to chelate divalent cations that could affect the stability of nucleic acids, while the boric acid provides buffering capacity at the desired pH range for optimal electrophoresis.

🧪 Applications
• Agarose gel electrophoresis: Used as a running buffer for DNA and RNA separation, especially effective for resolving smaller fragments of nucleic acids.
• Nucleic acid analysis: Widely employed in gel electrophoresis to analyze, separate, and purify nucleic acids like DNA and RNA.
• Molecular biology research: Commonly used in DNA sequencing, cloning, and restriction digestion analysis.
• DNA quantification and characterization: Facilitates accurate and high-resolution separation of DNA fragments for downstream applications like PCR analysis.