Potassium dichromate standard solution for absorbance according to Pharmacopeia
potassium dichromate standard solution is a reference reagent specifically prepared for the calibration and qualification of UV-Visible spectrophotometers in pharmaceutical and analytical laboratories. It ensures reliable and traceable absorbance measurements that comply with pharmacopoeial requirements and supports consistent analytical performance in quality control environments.
🏭⚗️ Production
This standard solution is prepared by dissolving an accurately weighed quantity of dried potassium dichromate (K₂Cr₂O₇) in dilute sulfuric acid, typically 0.005 M H₂SO₄, and diluting it to a specific volume. The resulting concentration provides stable absorbance values used for verifying spectrophotometric accuracy in accordance with USP, EP, and BP specifications.
🔬 Properties
The solution is an orange-colored transparent liquid that shows characteristic absorbance peaks at 235 nm, 257 nm, 313 nm, and 350 nm. It remains stable and reproducible when stored properly, making it a dependable reference for optical performance checks in laboratory environments.
🧪 Applications
In pharmaceutical and analytical laboratories, potassium dichromate standard solution is used for the calibration, qualification, and routine performance verification of UV-Visible spectrophotometers. It plays a critical role in ensuring the accuracy, linearity, and reproducibility of analytical results in drug quality control and research laboratories. The solution is employed in method validation studies, instrument qualification protocols (IQ, OQ, PQ), and periodic performance checks as required by Good Laboratory Practice (GLP) and Good Manufacturing Practice (GMP). Its use helps laboratories maintain regulatory compliance and ensures that spectrophotometric data remain accurate and reliable.
⚠️ Safety
Potassium dichromate is a toxic and carcinogenic compound containing hexavalent chromium and should be handled carefully using appropriate personal protective equipment such as gloves, lab coats, and goggles. The solution must be stored in amber glass containers, protected from light, and disposed of safely after neutralization according to environmental and pharmaceutical safety standards.
