Phosphoric acid for HPLC
| Formula | H3PO4 |
| Chemical formula | H3PO4 |
| Density | 1.71 g/cm3 (20°c) |
| HS Code | 28092000 |
| Storage | store above +15 °C |
| SDS | available |
| R phrase | R 34 |
| S phrase | S 26-36/37/39-45 |
| Odour | odourless |
| Form | liquid |
| Color | colourless |
| pH value | ~0.5 (100 g/l 20 °C) |
| Solubility in water | ( 20 ° C)soluble |
| Solubility in ethanol | ( 20 ° C)soluble |
| Melting point | 21 °C |
| Boiling point | 158 °C |
| Vapur pressure | 2 hPa (20°C) |
| Viscosity kinematic | 30.5 mPa*s (20°C) |
| Assay | ≥ | 85 | % |
| Description | Conforms | ||
| Solubility | Conforms | ||
| Identification | Conforms | ||
| Color | ≤ | 10 | Haze |
| Insoluble matter | ≤ | 10 | n |
| Aluminum | ≤ | 0/02 | ppm |
| Calcium | ≤ | 0/05 | ppm |
| Copper | ≤ | 0/025 | ppm |
| Iron | ≤ | 0/2 | ppm |
| Potassium | ≤ | 0/05 | ppm |
| Magnesium | ≤ | 0/02 | ppm |
| Sodium | ≤ | 0/1 | ppm |
| HPLC gradient suitability test (220,254 & | ≤ | 10 | ppm |
| 280 nm) chromophoric impurities on | ppm | ||
| 4-hydroxyacetophenon |
Phosphoric Acid for HPLC is a high‑purity, analytical‑grade reagent designed for use in pharmaceutical and analytical laboratories for the preparation of HPLC mobile phases, pH adjustment, and stabilizing chromatographic separations.
🏭⚗️ Production
Prepared from ultra-pure phosphoric acid with strict control over trace metal and organic impurities to ensure analytical-grade quality. Standardized for concentration, acidity, and stability. Supplied in chemically resistant, tightly sealed containers to prevent contamination, moisture uptake, or degradation.
🔬 Properties
A clear, colorless, highly viscous liquid with strong acidic properties. Exhibits excellent buffering capability in the low pH range, minimal UV absorbance suitable for HPLC detection, and high chemical stability under normal storage conditions. Highly corrosive to metals and tissues; reacts with strong bases and certain organics. Store in a cool, dry, well-ventilated area in suitable containers.
🧪 Applications
Widely used in pharmaceutical and analytical laboratories for preparing HPLC mobile phases, adjusting pH in aqueous or mixed solvent systems, and improving chromatographic reproducibility. Supports reversed-phase and ion-exchange separations with minimal interference. Solutions and equipment must be handled carefully to maintain high analytical purity.
⚠️ Safety
Strongly corrosive; can cause severe irritation or burns to eyes, skin, and respiratory tract. Use in a well-ventilated area with appropriate protective equipment including gloves, goggles, and lab coat. Avoid ingestion and contact with metals, bases, or oxidizers. Spills must be neutralized and cleaned up according to institutional and environmental safety guidelines. Store in a tightly closed, chemically resistant container in a cool, dry, well-ventilated area away from incompatible substances.
