Phosphate Saline Buffer (0.01 M,pH 7.5 )

Phosphate Saline Buffer (0.01 M, pH 7.5) is a dilute phosphate-buffered saline solution designed to maintain a slightly alkaline environment for various biological and biochemical applications.

🏭⚗️ Production
This buffer is prepared by dissolving sodium phosphate salts (monobasic and dibasic) along with sodium chloride in distilled water. The molarity of phosphate salts is adjusted to 0.01 M, and the pH is carefully set to 7.5, providing a stable buffering capacity. The solution is then sterilized, typically by filtration or autoclaving.

🔬 Properties
The solution is clear, colorless, and isotonic, with a stable pH of 7.5. It maintains ionic balance and prevents fluctuations in pH during experimental procedures, supporting biological sample integrity.

🧪 Applications
Commonly used in cell culture, molecular biology, and biochemical assays requiring a slightly alkaline environment. It serves as a washing buffer, reagent diluent, and medium for enzymatic reactions.

⚠️ Safety
Safe under normal laboratory conditions when handled properly. Use gloves and eye protection. Avoid ingestion and skin or eye contact. Dispose of waste according to institutional safety protocols.