Papanicolaou’s solution (EA 50)
| Density | 0.82 g/cm3 (20°C) |
| HS Code | 32041900 |
| Storage | 15 to 25 °C |
| SDS | available |
| R phrase | R 11-20/21/22-39/23 |
| S phrase | S 7-16-36/37- 45 |
| Odour | alcoholic |
| Form | liquid |
| Color | green – purpule |
| Solubility in water | soluble( 20 °C ) |
| Flash point | 17 °C |
| Description | Conforms | ||
| Identification | Conforms | ||
| Solubility | Conforms | ||
| Density | ~ | 0.82 | g/cm3 |
| Application test | Conforms |
Papanicolaou’s Solution (EA 50), also known as Eosin-Azure 50, is a staining solution primarily used in cytology and histology. This solution is a crucial part of the Papanicolaou stain (Pap stain), which is a common technique for examining the morphology of cells, especially in cervical smears (Pap smears) for cancer screening.
🏭⚗️ Production
Papanicolaou’s Solution (EA 50) is prepared by dissolving eosin, azure, and other dyes in specific proportions. The mixture is usually combined with ethanol and water, creating a solution that stains both cytoplasmic and nuclear components of cells.
🔬 Properties
The solution produces a pink to red color for the cytoplasm and a blue to purple color for the nucleus of cells, providing a clear contrast for microscopic examination. Papanicolaou’s solution works best at a slightly acidic pH, which helps differentiate the staining of cytoplasmic elements from the nuclear material. The dyes in this solution are soluble in alcohol and water, making it versatile for both alcohol-based and aqueous staining protocols.
🧪 Applications
Papanicolaou’s Solution (EA 50) is widely used in cytology for Pap smear testing, which helps detect abnormal cells and precancerous conditions such as cervical cancer. It is also used in histology to differentiate cell types in tissue samples, particularly epithelial cells. In diagnostic laboratories, EA 50 plays an essential role in exfoliative cytology to identify infections, inflammatory conditions, and neoplastic changes.
