Monosodium phosphate solution (1.0 ,pH 7)
Monosodium Phosphate Solution (1.0, pH 7) is an aqueous buffer solution made from monosodium phosphate, used to maintain a neutral pH environment in various biochemical and laboratory applications.
🏭⚗️ Production
This solution is prepared by dissolving a precise amount of monosodium phosphate (NaH₂PO₄) in distilled water to achieve a 1.0% concentration. The pH is adjusted to approximately 7.0 using sodium hydroxide or phosphoric acid as needed, ensuring a stable neutral environment.
🔬 Properties
The solution is clear and colorless, with a buffering capacity around pH 7. It stabilizes pH levels in biological and chemical assays, providing consistent experimental conditions. It is isotonic and compatible with many biological samples.
🧪 Applications
Commonly used as a buffer in molecular biology, biochemistry, and histology for enzyme reactions, cell culture media, and staining procedures where a neutral pH is critical. It helps maintain sample integrity during experimentation.
⚠️ Safety
Generally safe when handled properly. Use gloves and eye protection. Avoid ingestion and direct skin or eye contact. Follow laboratory safety protocols for disposal.
