Lactophenol solution
Lactophenol Solution is a mounting and staining reagent widely used in microbiology and mycology for preserving and visualizing fungal structures.
🏭⚗️ Production
Lactophenol Solution is prepared by mixing phenol, lactic acid, glycerol, and water in specific proportions. Phenol acts as a fungicidal preservative, lactic acid preserves fungal structures and clears tissue, glycerol provides a semi-permanent mounting medium, and water acts as the solvent. The solution is thoroughly mixed, filtered if necessary, and stored in a dark bottle at room temperature to maintain its properties.
🔬 Properties
Lactophenol Solution is a clear, viscous liquid that acts as both a preservative and mounting medium. It is miscible with water and stable under normal laboratory storage conditions. The phenol component provides antifungal activity.
🧪 Applications
This solution is commonly used for mounting fungal specimens for microscopic examination, preserving the morphology of fungal hyphae, spores, and other structures. It is widely used in clinical and environmental mycology.
⚠️ Safety
Handle with care; phenol is toxic and corrosive. Use gloves and eye protection, and work in a well-ventilated area or fume hood. Avoid ingestion, inhalation, and skin contact. Dispose of waste according to local chemical safety regulations.
