Lactophenol Cotton Blue

Lactophenol Cotton Blue is a staining reagent widely used in microbiology and mycology, primarily for highlighting fungal structures such as hyphae and spores.

🏭⚗️ Production
Lactophenol Cotton Blue is prepared by mixing methyl blue, a histological stain, with lactophenol—a solution of phenol, lactic acid, and glycerol in water. The methyl blue stains fungal cell walls a bright cerulean color, while lactophenol acts as a mountant.

🔬 Properties
Lactophenol Cotton Blue is a blue-colored staining solution that binds to chitin in fungal cell walls, providing strong contrast for microscopic observation. It is viscous, water-miscible, and stable under normal storage conditions.

🧪 Applications
This stain is primarily used in wet-mount preparations for visualization of fungal structures, especially in medical mycology.

⚠️ Safety
Handle with care; phenol is toxic and corrosive and can cause skin burns and respiratory irritation. Use gloves and eye protection, and work in a well-ventilated area. Avoid ingestion and inhalation. Dispose of any waste according to local chemical safety regulations.