Imidazole
| Formula | C3H4N2 |
| Chemical formula | C3H4N2 |
| Molar mass: | 68.08 g/mol |
| CAS number | 288-32-4 |
| Storage | Store at 15 to 25 ◦C |
| SDS | available |
| RTECS | NI3325000 |
| R phrase | R 22-34-63 |
| S phrase | S 22-26 -36/37/39-45 |
| Odour | amine like |
| Form | Solid |
| Color | Colorless to yellow |
| Ignition temperature | 480 ◦C |
| pH value | 10.5 (67 g/l 20 ◦C) |
| Solubility in water | 633 g/l (20 ◦C) |
| Solubility in ethanol | free soluble |
| Solubility in chloroform | soluble |
| Flash point | >135◦C |
| Boiling point | 256◦C |
| Melting point | 89 – 90◦C |
| Vapour pressure | 0.003 hp (20 ◦C) |
| Assay | ≥ | 99.0 | % |
| Description | Conforms | ||
| Identification | Conforms | ||
| Solubility | Conforms | ||
| UV/VIS Spectrum | Conforms | ||
| Melting point | 90 to 92 | ||
| Boiling point | ~ | 275.0 | |
| pH | 9.5 to 11.0 | ||
| Water | ≤ | 0.2 | % |
| Sulfated ash | ≤ | 0.1 | % |
| Iron | ≤ | 0.001 | % |
Imidazole is a high-purity, pharmacopeial-grade organic compound used in pharmaceutical and analytical laboratories as a buffering agent, catalyst, and reagent in biochemical and chemical synthesis applications.
🏭⚗️ Production
Produced under strictly controlled conditions to ensure high chemical purity, consistent particle size, and absence of residual solvents or impurities. Supplied in chemically resistant, tightly sealed containers to prevent contamination, degradation, and moisture absorption. Quality is verified according to pharmacopeial and analytical standards.
🔬 Properties
A white to off-white crystalline solid with mild amine-like odor. Soluble in water and many organic solvents. Exhibits amphoteric properties, acting as both a weak base and weak acid, and demonstrates excellent buffering capacity near neutral pH. Stable under proper storage conditions but hygroscopic and should be protected from moisture.
🧪 Applications
Used in pharmaceutical and analytical laboratories as a buffer component, catalyst, and ligand in coordination chemistry. Commonly employed in protein purification (such as His-tagged protein elution in IMAC), enzyme assays, and organic synthesis requiring mild basic or nucleophilic conditions.
⚠️ Safety
Irritating to skin, eyes, and respiratory tract. Handle with gloves, goggles, and protective clothing in a well-ventilated area or fume hood. Avoid inhalation of dust and contact with incompatible materials such as strong acids or oxidizers. Store in a cool, dry, tightly sealed container away from moisture and direct sunlight. Dispose of according to laboratory and environmental safety regulations.


