Hayem’S reagent (R.B.C. diluting fluid)

Hayem’s Reagent is commonly used in hematology as a red blood cell (R.B.C.) diluting fluid for counting the number of red blood cells in a blood sample. It is used in the hemocytometer method or automated blood cell counters for precise R.B.C. count determination.

🏭⚗️ Production
Hayem’s Reagent is prepared by dissolving sodium chloride (NaCl), sodium bicarbonate (NaHCO₃), and mercuric chloride (HgCl₂) in distilled water. This results in a solution that is isotonic, which prevents the cells from lysing during the dilution process.

🔬 Properties
Hayem’s Reagent has a clear or slightly yellow appearance and is isotonic with blood plasma, ensuring that red blood cells maintain their shape and integrity. The mercuric chloride in the solution helps to preserve the red blood cells, while sodium chloride and bicarbonate maintain the osmotic balance.

🧪 Applications
R.B.C. Counting: Hayem’s Reagent is primarily used for diluting blood samples to count the red blood cells in a hemocytometer or automated blood counter.
Microscopic Examination: The reagent is often used in manual counting procedures when a microscope is needed to visually count the red blood cells.
Blood Disorders: It helps in evaluating anemia, polycythemia, and other conditions affecting red blood cell count.