Formalin 10% fixative(TishoFix 10)
| Description | Conforms | ||
| Identification | Conforms | ||
| Solublity | Conforms | ||
| Appearance of solution | Conforms | ||
| Spec .rotation | _218 to -224 | ° | |
| Chloride | ≤ | 0.02 | % |
| Sulphate | ≤ | 0.03 | % |
| Heavy metals | ≤ | 0.001 | ° c |
| Iron | ≤ | 0.001 | % |
| Ammonium | ≤ | 0.02 | % |
| Ninhydrin positive substances | ≤ | 0.20 | % |
| Sulfated ash | ≤ | 0.10 | % |
| Loss on drying | ≤ | 0.50 | % |
| Assay | 98.5 to 101.0 | % |
| Description | Conforms | |
| Clarity | Conforms | |
| Application test | Conforms |
Formalin 10% Fixative (TishoFix 10) is a buffered aqueous solution of formaldehyde at approximately 4% concentration, designed for routine histological fixation of tissue specimens.
🏭⚗️ Production
TishoFix 10 is formulated by diluting concentrated formaldehyde (typically 37–40%) in water and buffering to achieve a neutral pH. The solution is standardized to contain 10% formalin by volume, which corresponds to about 4% active formaldehyde, ensuring consistent fixation performance across batches.
🔬 Properties
This fixative appears as a clear, colorless solution with a neutral pH around 6.9 to 7.1 and a density near 1.08 g/cm³. It forms cross-links between proteins, stabilizing cellular structures while preserving tissue morphology for histological examination. The solution remains stable at ambient temperature when properly sealed and protected from light and air.
🧪 Applications
TishoFix 10 is widely used in histology and pathology laboratories as a universal tissue fixative. It effectively preserves cellular and subcellular architecture, enabling reliable downstream processing such as embedding, sectioning, and staining. Its compatibility with diverse tissue types and workflows makes it a standard reagent for both routine and high-throughput histological analysis.
⚠️ Safety
This formalin-based fixative is toxic, potentially carcinogenic, and can release irritating vapors. It poses serious risks if inhaled or upon contact with skin or eyes. Proper handling requires the use of personal protective equipment including gloves, lab coat, and eye protection and processing within a well-ventilated space or fume hood. Safe storage in sealed containers away from heat and incompatible substances is essential.
