Ethanol for HPLC
| Formula | C2H60 |
| Chemical formula | C2H5OH |
| Density | ~ 0.79 g/cm3 (20°C) |
| Molar mass | 46.07 g/mol |
| CAS number | 64-17-5 |
| HS Code | 22071000 |
| EC number | 200-578-6 |
| Storage | at +15 to +25 °C |
| SDS | available |
| RTECS | KQ6300000 |
| R phrase | R 11 |
| S phrase | S 7-16 |
| Odour | alcohol like |
| Form | liquid |
| Color | colourless |
| Ignition temprature | 425 °C |
| p H | 7.0 (10g/l 20 °C ) |
| Solubility in water | soluble (20°C) |
| Solubility in | soluble (20°C) |
| Flash point | 12 °C |
| Melting point | ~-114 °C |
| Boiling point | 78.3°C |
| Vapur pressure | 59 hPa (20°C) |
| Viscosity dynamical | 1.2 mPa*s (20°C) |
| Saturation Concentration | 105 g/m3 (20°C) |
| Assay | ≥ | 99.9 | % |
| Description | Conforms | ||
| Identification | Conforms | ||
| Solubility | Conforms | ||
| Water | ≤ | 0.4 | % |
| Alkalinity | Conforms | ||
| Acidity | Conforms | ||
| Evaporation residue | ≤ | 2.0 | mg |
| Absorbance at 235 nm | ≤ | 5.0 | m AU |
| Absorbance at 254 nm | ≤ | 2.0 | m AU |
| Transmission at 225 nm | ≥ | 60.0 | % |
| Transmission at 240 nm | ≥ | 85.0 | % |
| Transmission from 260 nm | ≥ | 98.0 | % |
| Filterated by 0.2 μm filter |
Ethanol for HPLC is a high-purity, analytical-grade polar organic solvent widely used in pharmaceutical, biochemical, and analytical laboratories for HPLC, LC-MS, and sample preparation requiring minimal impurities and consistent chromatographic performance.
🏭⚗️ Production
Produced through controlled fermentation or synthetic routes followed by multi-stage distillation and purification to remove water, metals, organic impurities, and particulate matter. Subjected to fine filtration and stringent quality control to ensure consistent purity, low UV absorbance, and HPLC-grade specifications. Packaged in chemically resistant, airtight containers to prevent contamination, moisture uptake, or degradation during storage and handling.
🔬 Properties
A clear, colorless, volatile liquid with a characteristic alcoholic odor. Fully miscible with water and most polar organic solvents. Exhibits moderate polarity, low UV absorbance, and good chemical stability under recommended conditions. Reactive with strong oxidizers and certain acids. Store in a cool, dry, well-ventilated area in tightly sealed, chemically resistant containers, away from ignition sources.
🧪 Applications
Widely used as a mobile phase component in HPLC and LC-MS separations, providing reproducible retention times and peak shapes. Supports sample preparation, extraction, dilution, and chromatographic method development. Suitable for analytical workflows requiring high-purity solvents for sensitive detection techniques such as UV, fluorescence, or mass spectrometry. Ensures consistent and reliable chromatographic and spectrometric performance.
⚠️ Safety
Highly flammable and harmful if inhaled, ingested, or absorbed through the skin. May cause irritation to eyes, skin, and respiratory tract. Vapors can form explosive mixtures with air. Handle using appropriate PPE such as gloves, goggles, and lab coat in well-ventilated areas or fume hoods. Store away from heat, sparks, open flames, oxidizers, and incompatible chemicals in tightly closed, chemically resistant containers.
