Canada balsam
| Density | 0.98 g/cm3 |
| CAS number | 8007-47-4 |
| HS Code | 33013000 |
| EC number | 232-362-2 |
| Storage | at +5 to +30 °C |
| SDS | available |
| R Phrase | R 10 |
| Form | liquid |
| Flash point | 39 °C |
| Appearance | Conforms | ||
| Identification | Conforms | ||
| Solubility | Conforms | ||
| Refractive index | 1.515 – 1.530 | ||
| Acid value | 75 – 90 | ||
| Suitability for microscopy | Conforms |
Canada Balsam is a natural resin extracted from the balsam fir tree, widely used as a mounting medium in microscopy due to its excellent optical properties and adhesive qualities.
🏭⚗️ Production
Canada Balsam is obtained by tapping the balsam fir tree (Abies balsamea) to collect the resin, which is then purified through distillation and filtration processes to remove impurities. The purified resin is dissolved in solvents like xylene or toluene to prepare mounting media of varying viscosities.
🔬 Properties
Canada Balsam is a pale yellow to amber-colored viscous liquid or semi-solid resin with a refractive index close to that of glass (approximately 1.53). It is soluble in organic solvents but insoluble in water. The resin hardens upon drying, forming a transparent, durable, and adhesive film ideal for securing specimens under coverslips.
🧪 Applications
Canada Balsam is primarily used as a mounting medium in light microscopy to affix and preserve biological specimens on slides. Its optical clarity and refractive index improve image quality by reducing light refraction and scattering. It is also used in restoration and conservation of artworks due to its adhesive properties.
⚠️ Safety
Canada Balsam is generally safe in small amounts but contains solvents that are flammable and potentially harmful if inhaled or ingested. Use in a well-ventilated area with gloves and eye protection. Avoid skin contact and store away from heat and open flames.
