Ammonium dihydrogen phosphate for HPLC
| Formula | H6NO4P |
| Chemical formula | (NH4)H2PO4 |
| Molar mass | 115.03 g/mol |
| CAS number | 7722-76-1 |
| HS Code | 31054000 |
| EC number | 231-764-5 |
| Storage | Without limitation |
| SDS | available |
| Odour | odourless |
| Form | solid |
| Color | colourless |
| p H value | 3.8 – 4.4(50 g/l 20°C) |
| Solubility in water | 368 g/l (20°c) |
| Solubility in ethanol | sparingly soluble (20°C) |
| Melting point | 190 °C |
| Vapour pressure | 0.066 hPa(125 °C) |
| Thermal decomposition | >190 °C |
| Assay | ≥ | 99 | % |
| Description | Conforms | ||
| Solubility | Conforms | ||
| Identification | Conforms | ||
| Calcium | ≤ | 0.1 | ppm |
| Copper | 0/01 | ppm | |
| Iron | ≤ | 0/1 | ppm |
| Potassium | ≤ | 10 | ppm |
| Sodium | ≤ | 10 | ppm |
| HPLC gradient suitability test | ≤ | 10 | ppm |
| (220,254 & 280 nm) chromophoric | |||
| impurities on 4-hydroxyacetophenon |
Ammonium Dihydrogen Phosphate for HPLC is a high‑purity, analytical‑grade buffer salt specifically designed for use in pharmaceutical and analytical laboratories for preparing HPLC mobile phases, pH control, and enhancing chromatographic stability.
🏭⚗️ Production
Manufactured from ultra‑pure ammonium dihydrogen phosphate crystals dissolved in deionized water under rigorously controlled conditions to minimize trace metal and organic contaminants. Standardized for buffer capacity, molarity, and stability. Supplied in chemically resistant, tightly sealed containers to prevent contamination, moisture uptake, or degradation.
🔬 Properties
A white, crystalline powder highly soluble in water, forming a clear, colorless aqueous solution. Exhibits excellent buffering capacity in the pH range 3–5, low UV absorption suitable for HPLC detection, and high chemical stability. Non‑flammable under standard laboratory conditions but may react with strong bases or oxidizing agents. Store in a cool, dry, well‑ventilated area in suitable containers.
🧪 Applications
Used extensively in pharmaceutical and analytical laboratories for preparing HPLC mobile phases, adjusting pH in aqueous and mixed solvent systems, and improving reproducibility of chromatographic separations. Supports ion-exchange and reversed-phase HPLC while maintaining low background interference. Solutions and equipment should be handled carefully to preserve analytical-grade purity.
⚠️ Safety
Generally considered low hazard but can cause mild irritation to eyes, skin, and respiratory system. Use in a well-ventilated area with appropriate protective equipment including gloves and goggles. Avoid ingestion and contact with strong oxidizers or bases. Spills should be cleaned up following institutional and environmental safety guidelines. Store in a tightly closed, chemically resistant container in a cool, dry, well-ventilated area away from incompatible substances.
