هنکس

Hanks’ Balanced Salt Solution (HBSS) is an isotonic solution commonly used in cell culture and biological research. It contains a mixture of salts and glucose, and it helps maintain the physiological pH and osmotic pressure of cells, preventing them from undergoing stress.

🏭⚗️ Production
HBSS is typically prepared by dissolving a variety of salts, including sodium chloride (NaCl), potassium chloride (KCl), calcium chloride (CaCl₂), magnesium sulfate (MgSO₄), and glucose in distilled water. The solution is usually adjusted to a specific pH (around 7.4) to match physiological conditions.

🔬 Properties
Hanks’ Balanced Salt Solution is isotonic, meaning it has a similar osmolarity to that of body fluids, ensuring that cells do not shrink or swell when exposed to the solution. The solution is typically clear and colorless, and it can be supplemented with additional components such as glucose or antibiotics depending on the application.

🧪 Applications
Cell Culture: HBSS is primarily used in cell culture to wash cells, dilute cell suspensions, and maintain cell viability during experiments.
Tissue Culture: It is used in tissue culture to keep cells hydrated and maintain their integrity when transferred between environments.
Membrane Transport Studies: HBSS is often used in transport studies to evaluate the movement of substances across cellular membranes.
Washing and Dilution: It is used for washing cells to remove serum or other contaminants during experimental procedures.