Sodium hydroxide for HPLC
| Formula | HNaO |
| Chemical formula | NaOH |
| Density | 2.13 g/cm3 (20°C) |
| Molar mass | 40.0 g/mol |
| CAS number | 1310-73-2 |
| HS Code | 28151100 |
| EC number | 215-181-5 |
| Storage | Without limitation |
| SDS | available |
| RTECS | WB4900000 |
| R phrase | R 35 |
| S phrase | S 26-36/37/39-45 |
| Odour | oderless |
| Form | solid |
| Color | colourless |
| p H | ~14.0 (50g/l 20 °C) |
| Solubility in water | 1090 g/l (20°C) |
| Solubility in ethanol | 139 g/l (20°C) |
| Boiling point | 1390 °C |
| Melting point | 323 °C |
| Assay | 95.0 – 100.5 | % | |
| Description | Conforms | ||
| Solubility | Conforms | ||
| Identification | Conforms | ||
| Insoluble substances | ≤ | 0.2 | % |
| HPLC gradient suitability test | ≤ | 10 | ppm |
| (220,254,280 nm) chromophoric | |||
| impurities on 4-hydroxyacetophenon | |||
| K2CO3 (in solution 50%) | ≤ | 0/3 | % |
Sodium Hydroxide for HPLC is a high‑purity, analytical‑grade alkaline solution designed for use in pharmaceutical and analytical laboratories for the preparation of mobile phases, pH adjustment, and cleaning of HPLC instrumentation, glassware, and equipment.
🏭⚗️ Production
Prepared by dissolving ultra‑pure sodium hydroxide pellets or flakes in deionized water under strictly controlled conditions to ensure minimal trace metal content and absence of organic contaminants. Standardized for molarity, purity, and stability. Supplied in chemically resistant, tightly sealed containers to prevent contamination, moisture uptake, or reaction with atmospheric carbon dioxide.
🔬 Properties
A clear, colorless to slightly hygroscopic aqueous solution with strong alkalinity and high pH. Exhibits excellent solubility, stability, and minimal interference in chromatographic systems. Non‑flammable under normal laboratory conditions but highly corrosive and reactive with acids, metals, and certain organic compounds. Stable when stored in a cool, dry, well‑ventilated area in suitable containers.
🧪 Applications
Used in pharmaceutical and analytical laboratories for HPLC mobile phase preparation, pH adjustment of buffers, and cleaning of glassware, columns, and instrumentation. Ensures consistent chromatographic performance by maintaining proper pH and ionic strength. Suitable for aqueous and mixed solvent systems. Surfaces and equipment must be handled carefully to avoid contamination and preserve analytical-grade quality.
⚠️ Safety
Extremely corrosive and hazardous: can cause severe chemical burns, eye injury, and respiratory irritation. Use in a fume hood or well‑ventilated area with full protective equipment including chemical‑resistant gloves, goggles or face shield, protective clothing, and apron. Avoid contact with skin, eyes, acids, metals, and incompatible organic materials. Neutralize spills immediately and dispose of according to institutional and environmental safety regulations. Store in a tightly closed, chemically resistant container in a cool, dry, well‑ventilated area, away from incompatible substances and atmospheric carbon dioxide.
