Leishman’s Stain Solution
| CAS number | 12627-53-1 |
| HS Code | 3212 90 90 |
| Storage | Store at 15 to 30° c |
| SDS | available |
| Appearance | Dark Green to Very | ||
| Form | Dark Green | ||
| Identification | Powder | ||
| Color in methanol | Conforms | ||
| Solubility in methanol | Dark Blue to Very | ||
| Suitability for microscopy | Dark Blue | ||
| Version |
Leishman’s Stain Solution is a polychromatic staining reagent widely used for blood smear staining and differential leukocyte counting.
🏭⚗️ Production
Leishman’s stain is prepared by dissolving a mixture of methylene blue and eosin dyes in methanol, which acts as both solvent and fixative. The solution is carefully filtered to ensure clarity and stored in airtight containers to maintain stability.
🔬 Properties
The stain appears as a purple-blue liquid with strong staining properties. It contains both basic and acidic dyes, allowing it to differentially stain cellular components—nuclei appear blue to purple, while cytoplasm takes varying shades of pink or blue-green. The methanol content enables simultaneous fixation and staining of blood smears.
🧪 Applications
Leishman’s stain is primarily used in hematology to examine blood smears for the identification and differentiation of white blood cells, red blood cells, and platelets. It is widely employed in clinical laboratories for diagnosing blood disorders and infections like malaria.
⚠️ Safety
The solution contains methanol, which is toxic and flammable. Use gloves and eye protection when handling and work in well-ventilated areas. Avoid inhalation and prolonged skin contact, and store away from heat or open flames.
