Phosphate buffered saline (PBS)
| Density | 1.1 g/cm3 (20°C) |
| Storage | With out limitation |
| SDS | available |
| Odour | odourless |
| Form | liquid |
| Color | colorless |
| Solubility in water | soluble (20°C) |
| Turbidity | Conforms | ||
| Appearance | Conforms | ||
| Color | Conforms | ||
| p H | 7.0 – 7.8 | ||
| Performance test | Conforms | ||
| Heavy metals | ≤ | 5 | ppm |
Phosphate Buffered Saline (PBS) is a buffered saline solution commonly used in biological research, particularly in cell biology, biochemistry, and molecular biology. It is used for washing cells, diluting substances, and maintaining a stable pH environment during various experimental procedures.
🏭⚗️ Production
PBS is typically prepared by dissolving sodium chloride (NaCl), potassium chloride (KCl), disodium phosphate (Na₂HPO₄), and monosodium phosphate (NaH₂PO₄) in distilled water. The solution is adjusted to a desired pH (usually 7.4), which is close to the pH of human blood and tissues, to maintain physiological conditions.
🔬 Properties
PBS is an isotonic solution that helps maintain the osmotic balance of cells and tissues. The buffer components ensure the solution’s pH remains stable (around 7.4), which is essential for the proper functioning of enzymes and proteins during experiments. It is non-toxic and does not interfere with cellular processes, making it ideal for use in biological assays.
🧪 Applications
PBS is widely used for washing cells during cell culture, diluting reagents and antibodies, and preparing samples for microscopy or other analyses. It is also commonly used in immunoassays, such as Western blotting and ELISA, to maintain sample integrity. PBS is used in molecular biology procedures, such as the preparation of RNA and DNA, and in protein studies for ensuring the stability of biological macromolecules.
