EDTA Solution 0.1 M is a standardized aqueous solution of ethylenediaminetetraacetic acid (EDTA) with a molarity of 0.1 M, designed for analytical, laboratory, and pharmaceutical applications where precise chelation and metal ion titration are required.
🏭⚗️ Production
EDTA Solution 0.1 M is prepared by dissolving high-purity disodium EDTA in distilled or deionized water, followed by adjustment of pH using sodium hydroxide to ensure complete solubility and stability. The solution is carefully standardized against a primary standard, such as calcium carbonate or magnesium sulfate, to confirm exact molarity at 25°C. It is stored in tightly sealed, chemically resistant containers to maintain long-term stability and prevent contamination.
🔬 Properties
The solution is a clear, colorless liquid that is highly soluble in water. It acts as a strong chelating agent for divalent and trivalent metal ions and remains stable under normal laboratory conditions if protected from contamination and extreme pH fluctuations. EDTA Solution 0.1 M does not exhibit strong oxidizing or reducing properties and is compatible with most laboratory reagents.
🧪 Applications
In laboratory and pharmaceutical environments, EDTA Solution 0.1 M is commonly used in complexometric titrations to determine concentrations of metal ions such as calcium, magnesium, and zinc. It is also employed in quality control assays, formulation of pharmaceutical products, and preparation of standard solutions for analytical calibration.
⚠️ Safety
EDTA Solution 0.1 M is generally considered of low toxicity but may cause mild irritation to skin or eyes upon contact. Gloves and protective eyewear should be worn during handling. The solution should be stored in a cool, dry place, away from strong oxidizing agents and acids. Spills should be cleaned promptly, and disposal must follow laboratory safety protocols and local environmental regulations.